Clik here to view.
Breaking down fat: Composition of stromal vascular fraction
In the previous post we defined adipose-derived: stromal vascular fraction (SVF) versus stromal (stem) cells and highlighted difference between them. Today we will look at SVF more precisely and...
View ArticleBreaking down fat: Automated devices for adipose tissue processing
In the last decade, the process of cell isolation from adipose tissue was significantly refined. As part of commercialization strategy, developers invested in automation of the process. Currently...
View ArticleBreaking down fat: Considerations for adipose tissue processing
Quality and therapeutic potency of stromal vascular fraction (SVF) is very much depending on processing of adipose tissue. Cell composition of SVF can vary dramatically between different methods of...
View ArticleClik here to view.
Breaking down fat: Use of freshly isolated SVF versus cultured ASC
We defined Stromal Vascular Fraction (SVF) – as cell fraction, freshly isolated from native adipose tissue or liposuction aspirates and Adipose-derived Stromal (stem) Cells (ASCs) as plastic adherent...
View ArticleUsing molecular beacons for isolation of human osteogenic progenitors from...
Molecular beacons (MB) is a new technique for cell isolation, based on capture of genetic (intracellular or intranuclear) markers. MB have been used in research for isolation of embryonic stem cells...
View ArticleBreaking down fat: FDA’s position on regulation of adipose tissue-derived...
As response to accumulating complains about lack of clarity on regulation of human cell tissue products (HCT/P), derived from adipose tissue, FDA just released a guidance document, which clarifies...
View ArticleBreaking down fat: Comparison of devices for SVF isolation
For the routine clinical use of adipose tissue-derived stromal vascular fraction (SVF), automated commercial processing devices should be considered. These devices allow to save a lot of time, money,...
View ArticleBreaking down fat: Non-enzymatic “minimal” methods for SVF isolation
In the previous post of the series I asked if stromal vascular fraction (SVF) of adipose tissue could be isolated by simple centrifugation? The answer to this question could be a “game changer”,...
View ArticleClik here to view.
Breaking Down Fat: Trends in adipose cell-based clinical trials
As part of CellTrials project, I analyzed some trends in adipose cell-based clinical trials. The methodology is described here. I’d like to show you a snapshot of some data from the last four years....
View ArticleBreaking down fat: Quality control and release criteria for SVF-based products
Since FDA views adipose-derived stromal vascular fraction (SVF) as a “drug”, it is important to discuss quality control testing. Even if you will operate under different jurisdiction, where SVF is not...
View Article